![]() ![]() In this review, we discuss several issues of phage and phage-derived protein application approaches in therapy, diagnostics and biotechnology in general. ![]() There is also much interest in proteins encoded by lysis cassette genes (holins, endolysins, spanins) responsible for progeny release during the phage lytic cycle. Virm (Vimr and Troy, 1985) (Galli and Gerdes, 2010). There are at least five major groups of such enzymes – peptidoglycan hydrolases, endosialidases, endorhamnosidases, alginate lyases and hyaluronate lyases – that have application potential. Cultured under 50 g/ml kanamycin selection A K1F phage derivative. Encouraging performances were noted especially for phage enzymes involved in the first step of viral infection responsible for bacterial envelope degradation, named depolymerases. Moreover, the development of molecular biology and novel generation methods of sequencing has opened up new possibilities in the design of engineered phages and recombinant phage-derived proteins. Researchers have also investigated the application of non-lytic phages and temperate phages, with promising results. Many papers have been published proving the high antibacterial efficacy of lytic phages tested in animal models as well as in the clinic. The idea of using natural bacterial pathogens such as bacteriophages is already well known. Therefore, we are forced to develop an alternative or supportive treatment for successful cure of life-threatening infections. Nosocomial and community-acquired infections are usually caused by multidrug resistant strains. Currently, the bacterial resistance, especially to most commonly used antibiotics has proved to be a severe therapeutic problem. ![]()
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